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At Johns Hopkins University School of Medicine, I direct the Biomaterials and Drug Delivery Laboratory, serve on the executive committee of the Institute for NanoBioTechnology, and am associate director of the Translational Tissue Engineering Center. 

Vice Chair of the Drug Delivery Special Interest Group of the Society For Biomaterials.  

Previous and ongoing research on drug, gene and cellular therapies to treat brain tumors.

Areas of expertise: Drug delivery systems, Biomaterials, Nanotechnology, Non-viral gene therapy, Immunotherapy, Cell therapy. 

Tumor type: All pediatric brain tumors.

Existing collaborations relevant to drug delivery

Marjo Yliperttula (Professor of Biopharmaceutics, University of Helsinki, Finland). Collaborative research on the design of polymeric nanoparticles for gene delivery and analysis of polymer-DNA interactions.

Seung-Woo Cho (Associate Professor of Biotechnology, Yonsei University, Korea). Collaborative research on intracellular delivery of nanoparticles for cellular engineering. 

Publications relevant to drug delivery

Ben-Akiva E, Meyer RA, Wilson DR, Green JJ.  Surface Engineering for Lymphocyte Programming. Advanced Drug Delivery Reviews 2017; 114: 102-115

Rui Y, Quiñones G, Green JJ. Biodegradable and Bioreducible Poly (beta‐amino ester) Nanoparticles for Intracellular Delivery to Treat Brain Cancer. AIChE Journal 2017; 63(5): 1470-1482. (PMC4745093)

Kosmides AK*, Meyer RA*, Hickey JW, Aje K, Cheung KHN, Green JJ, Schneck JP.  Biomimetic Biodegradable Artificial Antigen Presenting Cells Synergize with PD-1 Blockade to Treat Melanoma.  Biomaterials 2017; 181: 16-26.(PMC5207804)(* indicates co-first authorship)  

Lima e Silva R*, Kanan Y*, Mirando AC, Kim J, Shmueli RB, Lorenc V, Fortmann S, Sciamanna J, Pandey NB, Green JJ, Popel AS, Campochiaro PA. Tyrosine Kinase Blocking Collagen IV-Derived Peptide Suppresses Ocular Neovascularization and Vascular Leakage.  Science Translational Medicine 2017; 9(373): eaai8030. (PMC5308917) (* indicates co-first authorship)  

Airan R*, Meyer RA*, Ellens N, Rhodes K, Farahani K, Pomper M, Kadam S, Green JJ. Noninvasive targeted transcranial neuromodulation via focused ultrasound gated drug release from nanoemulsions. Nano Letters 2017; 17(2): 652-659 (PMID: 28094959) (* indicates co-first authorship)  

Wilson DR, Mosenia A, Suprenant MP, Upadhya R, Routkevitch D, Meyer RA, Quinones‐Hinojosa A, Green JJ. Continuous Microfluidic Assembly of Biodegradable Poly (beta‐amino ester)/DNA Nanoparticles for Enhanced Gene Delivery. Journal of Biomedical Materials Research Part A 2017; 105(6): 1813-1825 (PMID: 28177587)

Zamboni C, Kozielski KL, Vaughan HJ, Nakata MM, Kim J, Higgins LJ, Pomper MG, Green JJ.  Polymeric nanoparticles as cancer-specific DNA delivery vectors to human hepatocellular carcinoma. Journal of Controlled Release 2017; 263: 18-28

Wilson DR, Routkevitch D, Rui Y, Mosenia A, Wahlin KJ, Quinones-Hinojosa A, Zack DJ, Green JJ.  A Triple-Fluorophore Labeled Nucleic Acid pH Nanosensor to Investigate Non-Viral Gene Delivery. Molecular Therapy 2017; 25(7): 1697-1709

Green JJ and Elisseeff JH.  Mimicking biological functionality with polymers for biomedical applications. Nature 2016; 540(7633): 386-394. (PMID: 27974772)

Kim J*, Mirando AC*, Popel, AS, Green JJ.  Gene delivery nanoparticles to modulate angiogenesis.  Advanced Drug Delivery Reviews 2016; doi: 10.1016/j.addr.2016.11.003 (PMID: 27913120) (* indicates co-first authorship)

Kozielski KL, Rui Y, Green JJ.  Non-viral nucleic acid containing nanoparticles as cancer therapeutics. Expert Opinion on Drug Delivery 2016; 13(10): 1475-1487. (PMID: 27248202)

Kim J, Kang Y, Tzeng SY, Green JJ.  PEG Synthesis and application of poly(ethylene glycol)-co-poly(β-amino ester) copolymers for small cell lung cancer gene therapy. Acta Biomaterialia 2016; 37: 120-130. (PMID: 27262740)

Bishop CJ, Liu AL, Lee DS, Murdock RJ, Green JJ.  Layer-by-Layer Inorganic/Polymeric Nanoparticles for Kinetically Controlled Multi-gene Delivery. Journal of Biomedical Materials Research A 2016; 104(3): 707-713. (PMC4852158)

Bishop CJ, Majewski RL, Guiriba TM, Wilson D, Bhise NS, Quinones-Hinojosa A, Green JJ.  Quantification of cellular and nuclear uptake rates of polymeric gene delivery nanoparticles and DNA plasmids via flow cytometry. Acta Biomaterialia 2016; 37: 120-130. (PMID: 27019146)

Mangraviti A, Tzeng SY, Gullotti D, Kozielski KL, Kim JE, Seng M, Abbadi S, Schiapparelli P, Sarabia-Estrada R, Vescovi A, Brem H, Olivi A, Tyler B, Green JJ, Quinones-Hinojosa A.  Non-virally engineered human adipose mesenchymal stem cells produce BMP4, target brain tumors, and extend survival. Biomaterials 2016; 100: 53-66. (PMID: 27240162)

Tzeng SY, Wilson DR, Hansen SK, Quinones-Hinojosa A, Green JJ.  Polymeric nanoparticle-based delivery of TRAIL DNA for cancer-specific killing. Bioengineering & Translational Medicine 2016; 1(2): 149-159. (PMC5365091)

Meyer RA and Green JJ.  Shaping the future of nanomedicine: Anisotropy in polymeric nanoparticle design.  WIREs Nanomedicine and Nanobiotechnology 2015; 8(2):191-207. (PMC4644720)

Kim J, Wilson DR, Zamboni CG, Green JJ.  Targeted polymeric nanoparticles for cancer gene therapy.  Journal of Drug Targeting 2015; 23(7-8): 627-641. (PMC4696040)

Bishop CJ, Tzeng SY, Green JJ.  Degradable polymer-coated gold nanoparticles for co-delivery of DNA and siRNA. Acta Biomaterialia 2015; 11:393-403 (PMC4289153)

Meyer RA, Sunshine JC, Perica K, Kosmides AK, Aje K, Schneck JP, Green JJ.  Biodegradable nanoellipsoidal artificial antigen presenting cells for antigen specific T-cell activation. Small 2015; 11(13): 1519-1525 (PMC4529071)

Meyer RA, Meyer RS, Green JJ.  An automated multidimensional thin film stretching device for the generation of anisotropic polymeric micro- and nanoparticles. Journal of Biomedical Materials Research (Part A) 2015; 103(8): 2747-2757 (PMC4535820)

Meyer RA, Sunshine JC, Green JJ.  Biomimetic particles as therapeutics.  Trends in Biotechnology 2015; 219: 488-99. (PMC4745091)

Mangraviti A*, Tzeng SY*, Kozielski KL*, Wang Y*, Jin Y, Gullotti D, Pedone M, Buaron N, Liu A, Wilson DR, Hansen SK, Rodriguez FJ, Gao G, DiMeco F, Brem H, Olivi A, Tyler B, Green JJ.  Polymeric nanoparticles for non-viral gene therapy extend brain tumor survival in vivo.ACS Nano 2015; 9(2): 1236-1249. (PMC4342728) (* indicates co-first authorship)          

Bishop CJ, Abubaker-Sharif B, Guiriba T-R, Tzeng SY, Green JJ.  Gene delivery polymer structure-function relationships elucidated via principal component analysis. Chemical Communications 2015; 51(60): 12134-12137. (PMC4568840)

Meyer RA and Green JJ.  Biodegradable polymer iron oxide nanocomposites: the future of biocompatible magnetism.  Nanomedicine 2015; 10(23): 3421-3425. (PMC4860811)

Bishop, C.J., Kozielski, K.L., Green, J.J.  Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles. Journal of Controlled Release 2015; 219: 488-499.(PMC4745091)

Sunshine JC*, Perica K*, Schneck JP, Green JJ.  Particle shape determines efficient CD8+ T cell activation and enhances tumor immunotherapy by artificial Antigen Presenting Cells. Biomaterials, 2014; 35(1): 269-277. (PMC3902087) (* indicates co-first authorship)              

Kim J, Sunshine JC, Green JJ.  Differential polymer structure tunes mechanism of cellular uptake and transfection routes of poly(β-amino ester) polyplexes in human breast cancer cells. Bioconjugate Chemistry 2014; 25(1): 43-51.  (PMC4016154).

Kozielski KL, Tzeng SY, Hurtado De Mendoza BA, Green JJ.  Bioreducible cationic polymer-based nanoparticles for efficient and environmentally triggered siRNA delivery to primary human brain cancer cells. ACS Nano 2014; 8(4): 3232-3241. (PMC4004313)

Guerrero-Cazares H*, Tzeng SY*, Young NP, Abutaleb AO, Quinones-Hinojosa A,  Green JJ.  Biodegradable polymeric nanoparticles show high efficacy and specificity at DNA delivery to human glioblastoma in vitro and in vivo. ACS Nano 2014; 8(5): 5141-5153. (PMC4046784) (* indicates co-first authorship)      

Wang T, Gilkes DM, Takano N, Xiang L, Luo W, Bishop CJ, Chaturvedi P, Green JJ, Semenza GL.  Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis. PNAS 2014; 111(31): E3234-E3242.  (PMC4128139) 

Tzeng SY and Green JJ.  Subtle changes to polymer structure and degradation mechanism enable highly effective nanoparticles for siRNA and DNA delivery to human brain cancer. Advanced Healthcare Materials 2013; 2(3): 468-480. (PMC3838886)

Shmueli RB, Bhise N, Green JJ.  Evaluation of polymeric gene delivery nanoparticles by nanoparticle tracking analysis and high-throughput flow cytometry. Journal of Visualized Experiments 2013; 73: e50176. (PMC3622088)

Kamat CD, Shmueli RB, Connis N, Rudin CM, Green JJ, Hann CL.  Poly(beta-aminoester) nanoparticle-delivery of p53 has activity against small cell lung cancer in vitro and in vivo.  Molecular Cancer Therapeutics 2013; 2(4): 405-415. (PMC3624031)

Tzeng SY, Higgins LJ, Pomper MG, Green JJ.  Biomaterial-mediated cancer-specific DNA delivery to liver cell cultures using synthetic poly(beta-amino ester)s. Journal of Biomedical Materials Research (Part A) 2013; 7: 1837-1845. (PMC3915782)

Bishop CJ, Ketola T-M, Tzeng SY, Sunshine JC, Urtti A. Lemmetyinen H, Vuorimaa-Laukkanen E, Yliperttula M, Green JJ.  The effect and role of carbon atoms in poly(beta-amino ester)s for DNA binding and gene delivery. Journal of the American Chemical Society 2013; 135(18): 6951-6957 (PMC3838887)

Kozielski KL, Tzeng SY, Green JJ.  Bioreducible linear poly(β-amino ester)s for siRNA delivery. Chemical Communications 2013; 49(46): 5319-5321. (PMC3894248)

Li C, Tzeng SY, Tellier L, Green JJ.  (3-Aminopropyl)-4-methylpiperazine end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based multilayer films for gene delivery.  ACS Applied Materials & Interfaces 2013; 5(13): 5947-5953. (PMC3838882)

Shmueli RB, Ohnaka M, Miki A, Pandey NB, Lima e Silva R, Koskimaki JE, Kim J, Popel AS, Campochiaro PA, Green JJ.  Long-term suppression of ocular neovascularization by intraocular injection of biodegradable polymeric particles containing a serpin-derived peptide. Biomaterials 2013; 34(30): 7544-7551. (PMC3838902)

Ketola T-M, Hanzlíková M, Leppänen L, Raviña M, Bishop CJ, Green JJ, Urtti A, Lemmetyinen H, Yliperttula M, Vuorimaa E.  ­­Independent vs. cooperative binding in polyethylenimine-DNA and poly(L-lysine)-DNA polyplexes. The Journal of Physical Chemistry B 2013; 117(36): 10405–10413(PMC3888923)

Kozielski KL*, Tzeng SY* and Green JJ.  siRNA nanomedicine: the promise of bioreducible materials.  Expert Review of Medical Devices 2013; 10(1): 7-10. (PMID: 23278217) (* indicates co-first authorship)      

Tzeng SY and Green JJ.  Therapeutic nanomedicine for brain cancer. Therapeutic Delivery 2013; 4(6): 687-704. (PMC3838881)

Sunshine JC and Green JJ.  Nanoengineering approaches to the design of artificial antigen presenting cells. Nanomedicine 2013; 8(7): 1173-1189 (PMC3951141)

Kozielski KL, Tzeng SY, Green JJ.  Bioengineered nanoparticles for siRNA delivery. WIREs Nanomedicine & Nanobiotechnology, 2013; 5(5): 449-468. (PMC3972625)

Bhise NS, Shmueli RB, Gonzalez J, Green JJ.  A novel assay for quantifying the number of plasmids encapsulated by polymer nanoparticles. Small 2012; 8(3): 367-373. (PMC3838880)

Shmueli RB*, Sunshine JC*, Zhenhua X, Duh EJ, Green JJ.  Gene delivery nanoparticles specific for human microvasculature and macrovasculature. Nanomedicine: Nanotechnology, Biology, and Medicine 2012;  8(7): 1200-1207. (PMC3350835) (* indicates co-first authorship)              

Tzeng SY, Hung BP, Grayson WL, Green JJ.  Cystamine-terminated poly(beta-amino ester)s for siRNA delivery to human mesenchymal stemcells and enhancement of osteogenic differentiation. Biomaterials 2012; 33(32): 8142-8151. (PMC3432640)

Sunshine JC, Peng DY, Green JJ.  Uptake and transfection with polymeric nanoparticles are dependent on polymer end-group structure, but largely independent of nanoparticle physical and chemical properties.Molecular Pharmaceutics 2012; 9(11): 3375-3383. (PMC3779641) 

Green JJ.  2011 Rita Schaffer Lecture: Nanoparticles for Intracellular Nucleic Acid Delivery.  Annals of Biomedical Engineering 2012; 40(7): 1408-1418. (PMC3380081)